Measurement of advanced glycation end products in the skin using autofluorescence spectroscopy in patients with chronic kidney disease
G.V. Papayan, A.M. Esayan, I.G. Kayukov, N.N. Petrischev, M.A. Yampolsky
1 SBEI HPE FSPbSMU n.a. Acad. I.P. Pavlov of RMPH, St. Petersburg; 2 Dialysis Center "Nefros", Krasnodar
Purpose. Determination of the diagnostic capabilities of use of the device «Skin-AGE» in patients with kidney disease.
Material and methods. Three groups of patients were evaluated: Group 1 (control group) consisted of practically healthy persons (47; F/M: 32/15, age: 16-78 years), Group 2 - renal transplant recipients (RTR; 24 persons; F/M: 13/11, age: 15-62 years), Group 3 – patients with end-stage renal failure (ESRF) on the permanent hemodialysis (79; F/M: 35/44, age: 22-82 years). Level of tissue accumulation of advanced glycation end products (AGEP) in the skin was estimated according to the intensity of autofluorescence (AF) using the device «Skin-AGE», developed in FSPbSMU n.a. Acad. I.P. Pavlov.
Results. Comparison of the data obtained using the device «Skin-AGE», with the results from healthy individuals using the known device «AGE Reader» (DiagnOptics, Netherlands), has showed that in both cases were similar age trends. The coefficient of correlation with age obtained using the device «AGE-reader» was 0.32 (P <0.05), and using our divice «Skin-AGE» - 0.74 (P <0.001). In Group 2 and Group 3, increase of the average values of AF was observed compared to the normal parameters by 1.75 ± 0.16 and 1.42 ± 0.16 times, respectively. In patients with ESRF, marked significant direct correlations of AF with the duration of dialysis (r = 0.36; P <0.0005), hemoglobin levels in peripheral blood (0.20; P <0.05) and calcium-phosphorus product (r = 0.24; p <0.025) were revealed. In RTR, a significant inverse relationship between AF and the duration of previous dialysis (r = -0.43; P <0.025), as well as direct correlation of AF with of total cholesterol levels (r = 0.36; P <0.05), low density lipoprotein cholesterol levels (r = 0,40; p <0.025) were revealed.
Conclusion. Measurements of tissue accumulation of AGEP according to AF levels using the device «Skin-AGE» can be used in the evaluation of patients with chronic kidney disease.
About the Autors
Information about the authors:
Papayan G.V. – PhD in Technical Sciences, Senior Resercher at the Center for Laser Medicine SBEI HPE FSPbSMU n.a. Acad. I.P. Pavlov of RMPH
Yesayan A.M. - MD, Professor, Head of the Department of Nephrology and Dialysis FPGE SBEI HPE FSPbSMU n.a. Acad. I.P. Pavlov of RMPH. E-mail: firstname.lastname@example.org
Kayukov I.G. - MD, Professor, Head of the Laboratory of Clinical Physiology of Kidney SRI of Nephrology SBEI HPE FSPbSMU n.a. Acad. I.P. Pavlov of RMPH
Petrishchev N.N. – MD, Head of the Center for Laser Medicine SBEI HPE FSPbSMU n.a. Acad. I.P. Pavlov of RMPH
Yampolsky M.A. – PhD in Medical Sciences, Chief Doctor of the Dialysis Center "Nefros", Krasnodar, Russia.